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Experiment 1: The Effect of Temperature on Catalase Activity
At which temperature did the catalase enzyme function at its best?
A. 40 °C
B. 21.5 °C
C. 80 °C
D. 10 °C
A
Experiment 1: The Effect of Temperature on Catalase Activity
At which temperature did catalase function at a slow rate?
A. 10 °C
B. 21.5 °C
C. 80 °C
D. 40 °C
B
Experiment 1: The Effect of Temperature on Catalase Activity
What caused the bubbles to form when you added the catalase to the hydrogen peroxide and water mixture at 40 °C?
A. Catalase activity heated the solution to its boiling point.
B. Hydrogen gas formed during the formation of
hydrogen peroxide.
C. The constant temperature bath heated the solution to its boiling point.
D. Oxygen gas formed during the decomposition of hydrogen peroxide.
D
Experiment 2: The Effect of Substrate Concentration on Catalase Activity
At which substrate concentration(s) did catalase function the longest?
A. the lowest substrate concentration
B. the highest substrate concentration
C. It
was equal for all the substrate concentrations.
D. the two middle substrate concentrations
B
Experiment 2: The Effect of Substrate Concentration on Catalase Activity
At which substrate concentration(s) did catalase function for the shortest amount of time?
A. the lowest substrate concentration
B. It was equal for all the substrate concentrations.
C. the two highest substrate
concentrations
D. the two middle substrate concentrations
A
Experiment 2: The Effect of Substrate Concentration on Catalase Activity
Why did the catalase function level off when different hydrogen peroxide concentrations were tested?
A. High concentrations of hydrogen peroxide raised the pH levels above the point of optimum catalase activity.
B. Catalase activity continually increased, so the
experiment must have been done incorrectly to produce results that appeared to level off.
C. High concentrations of hydrogen peroxide caused oxidative damage to the catalase enzymes and they were not able to perform at their optimum level.
D. At some point, all the catalase in solution was catalyzing reactions, so even if the hydrgen peroxide concentration was increased, the rate of the reaction could not increase any more.
D
Experiment 3: The Effect of pH on Catalase Activity
At which pH levels did catalase cease to function?
A. 6 and 10
B. 2 and 6
C. It worked at all pH levels.
D. 2 and 10
D
How much slower would hydrogen peroxide likely be decomposed if catalase was not available in cells?
A. 1,000,000 times
B. 100 times
C. 10 times
D. 1,000 times
A
Do the optimal conditions for catalase functioning that you found correspond with the conditions in the mammalian liver where it came from?
A. Yes, catalase functions best at the same low pH and temperature as occurs in the mammalian tissue.
B. No, catalase functions best at a much higher pH than in mammalian tissue and at a much lower temperature.
C. Yes, catalase worked best at a neutral pH and temperature of 40 °C, both
of which are close to mammalian tissue conditions.
D. No, catalase functions best at several very different pHs and temperatures, while the pH and temperature of mammalian tissue does not fluctuate.
C
Based on your results from this lab, would you expect the hydrogen peroxide you buy from the store to bubble like soda when you open it?
A. No, catalase sped up the rate of the reaction. Store bough
hydrogen peroxide will decompose into water and oxygen gas much too slowly for visible bubbles to form.
B. Yes, hydrogen peroxide is only stable in the presence of catalase. Without it, store bought hydrogen peroxide decomposes quickly.
C. No, hydrogen peroxide can only decompose into water and oxygen gas in the presence of catalase.
D. Yes, catalase had little effect on hydrogen peroxide decomposition. It would bubble quickly no matter what.
A
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